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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP753937Yeast1mgQuote
EP753937E. coli1mgQuote
BP753937Baculovirus200ugQuote
MP753937Mammalian Cell200ugQuote

Protein Information

SpeciesProtochlamydia amoebophila (strain UWE25)
UniProt IDQ6MAB7
Gene NamemiaB; Locus:pc1758
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMFSFNLLTQYIIMRSLKKFFVKTYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFN TCSIRDLAERKVMGKLGKLGLTKQSQAIIGVTGCMANAKKDSLFQKLPHIDFVLGTNNIH DLNHVLDEVLASGKQSIRTDDHFEFELDYLNAKREDQIKAYVSIIRGCDKFCTYCVVPYT RGSEVSRAPENILEECRHLVNQGYKEITLLGQNVNSYGKDKLEWKCLFHDLLYQLDKIPG LERVRFMTSHPVDISKELMEAIRDLKTLCEFVHFPLQAGSNRVLKKMHRIYTVEQYLEKV QMLKEIVPNVALGTDIIVGFPTETEEEFQETYRLLKEIEYSVAFLFSYSPRKGTPAMRWR DDVPEEVKQDRLQRLLQLQDTIYMKHRQAFLGQTVEVLVERRNFKDDRLVKGRTRCWKNV LFTGGDELVGTMQQVKIHGYSHQTLLGDLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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