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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP708129EAYYeast1mgQuote
EP708129EAYE. coli1mgQuote
BP708129EAYBaculovirus200ugQuote
MP708129EAYMammalian Cell200ugQuote

Protein Information

SpeciesEhrlichia ruminantium (strain Welgevonden)
UniProt IDQ5HBR5
Gene NamemiaB; Locus:Erum2620, ERWE_CDS_02650
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMQGLYIKSYGCQMNVYDSLIIENIIKPLGFSIVNEPSEADIVILNTCHIREKAAEKLYSE LGRIRKIQETKNLTIVVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKARKIKK QIINIDFPVISKFDAIAVEEYTKNQKVSAFISVQEGCNKFCSFCVVPYTRGEEYSRTVEA IFKEALILADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYYTTSHP RDMHESLYEAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKHRSDIA YSSDFIVGFPGETDEDFENTIQLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDEIKSQR LTKLQELVHKQQLEFNKKMIGETHPVLFYKKGKFDNQIIGKTPYMQSCYINTENPDLYYN KIVPIKITDAHKNHLTGIIPNTLPV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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