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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP685952Yeast1mgQuote
EP685952E. coli1mgQuote
BP685952Baculovirus200ugQuote
MP685952Mammalian Cell200ugQuote

Protein Information

SpeciesWolbachia sp. subsp. Brugia malayi (strain TRS)
UniProt IDQ5GSS2
Gene NamemiaB; Locus:Wbm0364
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMKSLYIKTYGCQMNVYDSILMENIIKPLGFNVVNDVEKADLVILNTCHIREKAAEKLYSE LGKIHSSRKNKEITIVAAGCVAQAEGEEIFRRAPFVDIVVGPQSITTLPELIVKASRSKG HVINTDFPKVMKFDRLSDECYGNSQGSSAFLSIQEGCDKFCTFCVVPYTRGAEYSRPVNE IFREALKLVANGAKEINLLGQNVNAYHGEYEGEVWDLGKLISHIAKIEKLERIRYTTSHP RDMHESLYLAHAEAPKLMPFIHLPVQSGSNKILRAMNRKYTTEEYLEIIERFRKLKPKIE FSSDFIVGFPGETEKDFEGTIKLVERVRYAQAYSFKYSTRPGTPGAERKDQVPEKVKTER LLHLQKLINKQQLEFNQSMVGKTIPVLFSNKKGKHQNQIIGKSPYMQSVCIDDPEDKCRD KIVNVRILEAWQNSLLGRELQE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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