Request QuoteCatalog Number: xP714644BQUSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP714644BQUYeast1mgQuote
EP714644BQUE. coli1mgQuote
BP714644BQUBaculovirus200ugQuote
MP714644BQUMammalian Cell200ugQuote

Protein Information

SpeciesBacillus licheniformis (strain DSM 13 / ATCC 14580)
UniProt IDQ65JE5
Gene NamemiaB; Locus:BLi01925, BL03660
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMNEKQRIESGQVNPSDKKSEKDYSKYFEAVYVPPSLKDAKKRGKEEVKYHNDFKISEQFR GMGEGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDSTEDANVILLNTCAIRENAENK VFGEIGHLKALKKDNPDLILGVCGCMSQEESVVNRILKKHPFVDLIFGTHNIHRLPELLS EAYLSKEMVIEVWSKEGDVIENLPKVRTGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR RPEDIIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKIDIPRIRFTT SHPRDFDDHLIEVLAKGGNLLDHIHLPVQSGSSDVLKLMARKYDRERYLELVAKIKKAMP NASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVK KERLQRLNEVVNEISAKKMKEYEGQVVEVLVEGESKNNPDILAGYTRKNKLVNFKGPKEA IGQLVNVKIHQAKTWSLDGEMVGEAIEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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