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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP734687RNEYeast1mgQuote
EP734687RNEE. coli1mgQuote
BP734687RNEBaculovirus200ugQuote
MP734687RNEMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia typhi (strain ATCC VR-144 / Wilmington)
UniProt IDQ68VU1
Gene NamemiaB; Locus:RT0795
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLYIKTYGCQMNVYDSVKIQDLLYPFGYESTEDIKEADIIILNTCHIREKAAEKTYS ELGRIKKLQNTRKQEGLNPAIIVVAGCVAQAEGEVIFTRTPYVDIVVGPQSYYNLPELIS KVVRHEKHLIDLDFVEEAKFDNLPEQLYTQGASSFISVQEGCDKFCTFCVVPYTRGAEFS RSVEQVYRESLKVVSNDTKEIILLGQNVNAYHGKGPKDKIFTLADLLKHLAQIPNLERLR YMTSHPIDMTDDLIKLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDRDYYFNIINRLRE ARSDIVLSSDFIVGFPGETEKDFEDTLDLVQRVKYGQCYSFKYSPRPGTPGAIRTDQIPE HIKSKRLTILQKELATQQLAFNESCVGSTMKVLFDRDGKFEDQIIGKTPYMQSVYIHNPN KSLLGKIVDVIITKAALNSLTGEIL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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