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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP719942Yeast1mgQuote
EP719942E. coli1mgQuote
BP719942Baculovirus200ugQuote
MP719942Mammalian Cell200ugQuote

Protein Information

SpeciesNocardia farcinica (strain IFM 10152)
UniProt IDQ5YT08
Gene NamemiaB; Locus:NFA_38350
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-503
Expression Tag6xHis
Purity>90%
AA SequenceMTRARSYEIRTFGCQMNVHDSERLSGLLEDAGYVKAAPGATADLVVFNTCAVRENADNKL YGTLGHLAPVKAGRPGMQIAVGGCLAQKDRDTVVRKAPWVDVVFGTHNIGSLPVLLERAR HNEQAQVEILESLEAFPSTLPAKRESAYAGWVSISVGCNNTCTFCIVPALRGKEVDRRPG DVLAEVQALVDQGVLEVTLLGQNVNSYGASFADPDEPRDRGAFAKLLRACGGIEGLERVR FTSPHPAEFTDDVIEAMAQTPNICPQLHMPLQSGSDRVLKAMRRSYRKARYLGIIEKVRA AMPHAAITTDIIVGFPGETEEDFQETLDVVRQARFTSAFTFQYSKRPGTPAAEMADQVPK QVVQERYDRLIALQEEISLAANRELIGTEVELLVAEGSGKKNAATARMSGRARDGRLVHF RPGGTAEPIRPGDLVTVDITEAAPHHLIADAPVKTHRRTRAGDAHERGITPKTAPIGVGL GLPRIGAPAPEPVAAGCSTGCGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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