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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP714571BDQYeast1mgQuote
EP714571BDQE. coli1mgQuote
BP714571BDQBaculovirus200ugQuote
MP714571BDQMammalian Cell200ugQuote

Protein Information

SpeciesBacteroides fragilis (strain YCH46)
UniProt IDQ650P7
Gene NamemiaB; Locus:BF0028
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMNELTGADFKSATADDNKKLFIETYGCQMNVADSEVIASVMQMAGYSVAETLEEADAVFM NTCSIRDNAEQKILNRLEFFHSMKKKKKHLIVGVLGCMAERVKDDLIEHHHVDLVVGPDA YLTLPELIASVEAGEKAMNVELSTTETYRDVIPSRICGNHISGFVSIMRGCNNFCTYCIV PYTRGRERSRDVESILNEVADLVSKGYKEITLLGQNVNSYRFEKEGGEVVTFPMLLRLVA EAAPGIRVRFTTSHPKDMSDETLEVIAQVPNVCKHIHLPVQSGSSRILKLMNRKYTREWY LDRVAAIKRIVPDCGLTTDIFSGFHSETEEDHRESLSLMEACGYDAAFMFKYSERPGTYA SKHLEDNVPEEIKVRRLNEIIALQNRLSAESNNRCIGKTYEVLVEGVSKRSRDQLFGRTE QNRVVVFDRGTHRIGDFVNVRITEASSATLKGEEVFS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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