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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP726686MFMYeast1mgQuote
EP726686MFME. coli1mgQuote
BP726686MFMBaculovirus200ugQuote
MP726686MFMMammalian Cell200ugQuote

Protein Information

SpeciesMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
UniProt IDQ608N1
Gene NamemiaB; Locus:MCA1459
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-458
Expression Tag6xHis
Purity>90%
AA SequenceMPQKLYIETFGCQMNEYDSAKMRDLLEVSDSFELARSPEEADVLLLNTCSIRDKAQEKVF SQLGRWRPIKLRRPEVVIGVGGCVASQEGEALQKRAPYVDIVFGPQTLHRLPSMLEQVRC ERRPVVDVSFPAIEKFDALPEPRADGPKAFVSVMEGCGKYCTFCVVPYTRGEEISRPVDD VIAEIVALAEQGVREVNLLGQNVNAYRGVLADGGMADLALLMHYVAAVDGIDRIRFTTSH PVEFSDALIEAFRDIPQLVSHLHLPVQSGSDRILRLMKRGHTRAEYMAKVAKLREIRPDL SLSSDFIVGFPGETDEDFEDTMALIEQLGFDQSFSFIFSARPGTPAAAMADDVPPETKRA RLARLQAKIADNAAKIGASMVGSIQSVLVEGTSRKNFNELSGRTENNRVVNFAGHPRLIG QFVDVVITESLPNSLRGRLIGVAHESDFISPDSTAQIA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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