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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP728063Yeast1mgQuote
EP728063E. coli1mgQuote
BP728063Baculovirus200ugQuote
MP728063Mammalian Cell200ugQuote

Protein Information

SpeciesDesulfotalea psychrophila (strain LSv54 / DSM 12343)
UniProt IDQ6ALW9
Gene NamemiaB; Locus:DP1927
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMEQRSFFIKTYGCQMNLRDSEIIAQILNNNGYVETSEIGGADLVLLNTCSIRAKAEQKVM SKLGELRRNKKINPRMQICVAGCVAQQEGKQIQAKMPHVDLVIGTQYIYAINELLERSRT EGPITATNLDDKYVIPQFIPETTGKEHEGEFRKFVTIMQGCNNFCTYCVVPYTRGREVSR SIKDIVEEITVLVKSGIKEITLLGQNVNSYAQTNTVTEDDTPATFSDLLRQVAAVEGLKR LRFTTSNPKDLSNDLMQCFKDLDVLCPQFHLPVQAGSNKVLKEMGRKYTVESYLDLVTQL RENCPEIAITTDIIVGFPGETDEEFEETMKMLETVRYHGSFSFKYSDRPGTKANELTNKV DESVKSARLARFQARQDEIGLERNQEYIGTTQEVLIEELRDGEIKGRMGTNHIVHAIGLT NKKPGDFLMAHVTAAGQHSLRGSIVEE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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