Request QuoteCatalog Number: xP719378TNTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP719378TNTYeast1mgQuote
EP719378TNTE. coli1mgQuote
BP719378TNTBaculovirus200ugQuote
MP719378TNTMammalian Cell200ugQuote

Protein Information

SpeciesThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
UniProt IDQ5SME9
Gene NamemiaB; Locus:TTHA1308
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-465
Expression Tag6xHis
Purity>90%
AA SequenceMPPCYPFLLSWEGWARDPPGRPGGAMRAHIITYGCQMNEYDSHLVASELVSLGWELVDSV EEADFVLVNTCAVRGKPVEKVRSLLGQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGV DVLLGPGALTSLPEALKANERFWDLTFREDVLDYIPPPPKGALSAHVTIIRGCNHHCTYC IVPTTRGPEVSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVG GMGIPRVRFLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYL ERIRKIREALPDAVLSTDIIVGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAY KHFQDLPREVKVERLMRLIEKQKEWSYRRNLEWVGKTVEVLVRGEAKEEGFVQGHDRGNH PVLVPASQAPVPGLYQVEIKQATPHLLFGEVVGAEAPAPIPLPVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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