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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP689659FPZYeast1mgQuote
EP689659FPZE. coli1mgQuote
BP689659FPZBaculovirus200ugQuote
MP689659FPZMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
UniProt IDQ5N199
Gene NamemiaB; Locus:syc1731_c
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-452
Expression Tag6xHis
Purity>90%
AA SequenceMSTPRRYHITTFGCQMNKADSERMAGILEDLGYIWSEEANDADLVLYNTCTIRDNAEQKV YSYLGRQAERKRQQPDLTLIVAGCVAQQEGESLLRRVPELDLVMGPQHANRLADLLAQVE AGSQVVATEEVEIAEDITQPRRDSTITAWVNVIYGCNERCTYCVVPNVRGREQSREPAAI RAEIEQLAAQGYREITLLGQNIDAYGRDLPGSTPEGRHLHTLTDLLYTIHDVPGIERIRF ATSHPRYFTERLIRACAELPKVCEYFHIPFQSGDNDVLKAMARGYTVERYLRIVEQIRDI IPDAAISADAIVAFPGETEEQFENTLKLVEQVGFDLVNTAAYSPRPGTPAANAPNQLSEE VKQDRLQRLNHLVAQMAADRSQRYLGRTEEVLIEATNPRNPQQVMGRTRTNRLVFCDGTI AQLEGQLVPVRITETRAFSLTGQILSPVAAGC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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