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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP660177Yeast1mgQuote
EP660177E. coli1mgQuote
BP660177Baculovirus200ugQuote
MP660177Mammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain CC9902)
UniProt IDQ3AV90
Gene NamemiaB; Locus:Syncc9902_1540
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-472
Expression Tag6xHis
Purity>90%
AA SequenceMTATSLKTLTPPSKQGLDQNRRSYWITTFGCQMNKADSERMAGILESMGYCEATAELDAD LVLYNTCTIRDNAEQKVYSYLGRQAQRKRDNPNLTLVVAGCVAQQEGESLLRRVPELDLV MGPQHANRLETLLQQVDSGQQVVATEEHHILEDITTARRDSAICGWVNVIYGCNERCTYC VVPSVRGQEQSRRPEAIRLEMEGLAAQGFKEITLLGQNIDAYGRDLPGITAEGRREHTLT DLLHHVHDVEGIERLRFATSHPRYFTERLIDACADLSKVCEHFHIPFQSGDNALLKSMAR GYTVERYRRIIDRIRDRMPDASISADVIVGFPGETDAQYRRTLDLIDEIAFDQVNTAAYS PRPNTPAATWDNQLPESVKVERLKEINALVERNARERNIRYQGRTEEVLAEGINPKDPEQ LMGRTRTNRLTFFSATSPDGHLYQPGDLVNVRIDAVRSFSLSGTPLPSNALH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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