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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP675512Yeast1mgQuote
EP675512E. coli1mgQuote
BP675512Baculovirus200ugQuote
MP675512Mammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
UniProt IDQ49X85
Gene NamemiaB; Locus:SSP1468
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-513
Expression Tag6xHis
Purity>90%
AA SequenceMNEEQRKSTSIDILAERDKKTKDYSKYFEHVYQPPSLKEARKRGKEEVNYNDDFQIDEKY RNMGQGKTFLIKTYGCQMNAHDTEVMAGILGALGYTPTEDINHADVILINTCAIRENAEN KVFSEIGNLKHLKKEKPETVIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHRLPEIL EEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKERS RRPEDIIDEVRDLARQGYKEITLLGQNVNAYGKDIDGLAYGLGDLLEDISKIDIPRVRFT TSHPWDFTDRMIEVIANGGNIVPHVHLPVQSGNNAVLKIMGRKYTRESYLDLVNRIKTHI PNVALTTDIIVGYPNETDEQFEETLTLYDEVEFEHAYTYIYSQRDGTPAAKMNDNVPLDV KKDRLQQLNKKVACYSERAMQQYEGQTVQVLCEGVSKKDDTVLSGYTSKNKLVNFKAPKS MIGKIVNVYIDEAKQFSLNGTFISVNDKTVVTQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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