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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP700300XAFYeast1mgQuote
EP700300XAFE. coli1mgQuote
BP700300XAFBaculovirus200ugQuote
MP700300XAFMammalian Cell200ugQuote

Protein Information

SpeciesXanthomonas campestris pv. campestris (strain 8004)
UniProt IDQ4UVS2
Gene NamemiaB; Locus:XC_1788
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-485
Expression Tag6xHis
Purity>90%
AA SequenceMPGTSVSDLPTTATAVDAPALLPLPVGRPQAPALVRGKLYIKTHGCQMNEYDSAKMADVL AASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVAS QEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRADG PSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAY RGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANY LHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFDKT MKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDAVKQARLARLQAHINAHAAGISQRMV GSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNPRLVGQFVDVLITEALSNSLRGRIQL DDSAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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