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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP651790Yeast1mgQuote
EP651790E. coli1mgQuote
BP651790Baculovirus200ugQuote
MP651790Mammalian Cell200ugQuote

Protein Information

SpeciesHahella chejuensis (strain KCTC 2396)
UniProt IDQ2SBF4
Gene NamemiaB; Locus:HCH_05348
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMAQKLFIKTYGCQMNEYDSSRMADLLGESHSVELTDNPDEADILLLNTCSIREKAQEKVF HQLGRWKTLKEKNPNLLIGVGGCVASQEGDAIRDRAPYVDMVFGPQTLHRLPEMVNSVAH QKIPMVDVTFPEIEKFDRLPMPSSEGASAFVSIMEGCSKYCTFCVVPYTRGEEVSRPVDD VIAEIAHLAGQGVREVNLLGQNVNAYRGLTHDGDYMDLAELITYVASVDGIDRIRYTTSH PVEFSDSLIDVYASVPKLVSHLHLPVQSGSDRILNLMKRGHTVAEYTDKLRRLQEIRPDI SLSSDFIVGFPGETDADFEETMNLINEIGFDHSFSFVYSARPGTPAANLEDNVSEEAKKQ RLAILQQRILQFAQDISRKMVGSTQRILVTGVSKKDPGELQGRTENNRVVNFRSDSHDII GRFVDVTITAALPNSLRGERVDSLDY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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