Request QuoteCatalog Number: xP661373CDJSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP661373CDJYeast1mgQuote
EP661373CDJE. coli1mgQuote
BP661373CDJBaculovirus200ugQuote
MP661373CDJMammalian Cell200ugQuote

Protein Information

SpeciesCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
UniProt IDQ3ACA4
Gene NamemiaB; Locus:CHY_1398
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-440
Expression Tag6xHis
Purity>90%
AA SequenceMAEKSYYIITHGCQMNVHDSETIAGILESMGFVPSPEEKTADLIIINTCSVRETAENKVF TKIGELKKLKRENPDLVIGVGGCIPQQEKVAKRLLERFPHLDFIFGTHNLPELPKILERV FEKHERVLEVWQSEGQIVEGIPVKREPGVRAWVTIMYGCNNFCTYCIVPYVRGRERSRKK EDILQEIRQLVAEGYREVTLLGQNVNSYGKDLKGKPMFAELLADIEKIDGLWRVRFTTSH PRDLTDDVIEVMASSRKICEHLHLPVQAGSNKILKAMHRGYTREYYLNLVEKIRAKIPKV SFTTDIIVGFPGETEEDFEQTLDLVRKVRYDSAFTFVYNKRTGTPAAEMKDQVPEEVKSR RIQELIELQNGISLELNKNEEGNIHEILVEGKSKTDETKLAGRTRTNKLVVFNGNEDLVG KLVKVKITEGKLFHLEGVLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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