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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP660034Yeast1mgQuote
EP660034E. coli1mgQuote
BP660034Baculovirus200ugQuote
MP660034Mammalian Cell200ugQuote

Protein Information

SpeciesPelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
UniProt IDQ3A594
Gene NamemiaB; Locus:Pcar_1214
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-438
Expression Tag6xHis
Purity>90%
AA SequenceMKSFYLETFGCQMNVVDSEQIVGLVQSLGYSSVDSPEQANLIILNTCSIRARAERKVYGH LGRFKPLKQRRPELIIAVCGCVAQQEGQRMLEKVPYLDIVCGTHNIHRLADMVRDAELHR ARHVEVDFLEADKRRRLFPERAPSAEVSRFVTVIQGCDNFCSYCIVPHVRGREVSRPSAE VLEEVRLLVEQGAREITLIGQNVNSYGCKEDDEISFASLLRKVAEVDGLERIRFMTSHPK DLSDELIDCFADLDKLCKHIHLPVQAGGDAVLKAMRRGYTRDQYLGRIERLRRVCPEIRM TSDVIVGFPGETESEFEQTMDLLERARFTEIYSFIFSARPGTSAADLPDDIPKEVKQQWF DRMLALQEEITRQYHQMDIGQVLPVLVEGSSRQGNGQLFGRTTWNRIVNFDGNPDLVGRI VPVRLTVAYRNSHLGERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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