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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP661589Yeast1mgQuote
EP661589E. coli1mgQuote
BP661589Baculovirus200ugQuote
MP661589Mammalian Cell200ugQuote

Protein Information

SpeciesXanthomonas campestris pv. vesicatoria (strain 85-10)
UniProt IDQ3BS94
Gene NamemiaB; Locus:XCV2638
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-484
Expression Tag6xHis
Purity>90%
AA SequenceMPGTPVSDLSAAAAVDAPALLPLPVARPSAPAVVRGKLYIKTHGCQMNEYDSAKMADVLA ASEGLELTDNPEEADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQ EGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRAEGP SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYL HLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETEADFEKTM KLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPEAVKQARLARLQAHINAHAASISQSMVG SVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD DSAQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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