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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP671169Yeast1mgQuote
EP671169E. coli1mgQuote
BP671169Baculovirus200ugQuote
MP671169Mammalian Cell200ugQuote

Protein Information

SpeciesPsychrobacter arcticus (strain DSM 17307 / 273-4)
UniProt IDQ4FQU4
Gene NamemiaB; Locus:Psyc_1766
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-496
Expression Tag6xHis
Purity>90%
AA SequenceMSVTVFNPRIDDAAASDITAVTPAVTALNELSSAQAPVKSATKKVFVTTQGCQMNVYDSG KMLDVLGDSHGMEVTHDIDEADVLLMNTCSIREKAQEKVFSELGRWRKLKEKRPDLVIGV GGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDQSHQQREIAPKNRIGTVDVSFPSI EKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAAQGV REINLLGQNVNGYRGEKDDGNICRFAELLHYVSHVDGVERIRYTTSHPLEFTDDIIDAYA QLPELVSHLHLPVQSGSNTILAAMKRNHTIDVYINQINKLKAIRPDIHLSSDFIIGFPGE TDQDFQDTLTLAKELNFDHSYSFIYSKRPGTPAAELPDDVSFKTKKERLAEFQKVIIDST LAKTHEMVGTTTRVLVEQVANRHPDCLIGTADNTRTVMFPYAVDKMDELLGKIVSVRITD FVSPHMVKGEIEAVLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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