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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP675211Yeast1mgQuote
EP675211E. coli1mgQuote
BP675211Baculovirus200ugQuote
MP675211Mammalian Cell200ugQuote

Protein Information

SpeciesThermobifida fusca (strain YX)
UniProt IDQ47RR8
Gene NamemiaB; Locus:Tfu_0811
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-494
Expression Tag6xHis
Purity>90%
AA SequenceMSQSRTYQVRTYGCQMNVHDSERLSGLLEAAGYQRAAEGTTPDVVVFNTCAVRENADNRL YGNLGQLRPVKDANPDMQIAVGGCLAQKDRGEIIRRAPWVDVVFGTHNIGSLPALLERAR VQREAQVEIVESLERFPSTLPTRRESVYAAWVAISVGCNNTCTFCIVPALRGKEKDRRPG DILAEIRAVVAEGAIEVTLLGQNVNAYGSEFGDRQAFSKLLRACGEIEGLERVRFTSPHP RDFTDDVIAAMAETPNVMPQLHMPLQSGSDRILRAMRRSYRAERFLRIVEKVREAIPDAA ITTDVIVGFPGETEEDFAETLRVMEEVRFAHAFTFQYSKRPGTPAASMDNQVPREVVKER YQRLLELQERISEEENAKFVGREVEVLVAEGEGRKDDVHRRMSGRARDNRLVHFAVDEGV TVRPGDMVTVEVTYAAPHHLVADSGIRNLRRTRAGDAWEARNAPERRPTGVLLGMPKVGA PEPQPSVVGGCCDS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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