Request QuoteCatalog Number: xP684018RMTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP684018RMTYeast1mgQuote
EP684018RMTE. coli1mgQuote
BP684018RMTBaculovirus200ugQuote
MP684018RMTMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
UniProt IDQ4UK06
Gene NamemiaB; Locus:RF_1278
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEDADVIILITCHIREKAAEKTYS ELGRIKKLQDTRKKQGLNSAIIVVAGCVAQAEGEEIFSRAPYVDIVVGPQSYYNLPELIS KVVRHEKHLIDLDFVEEAKFDNLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFS RNVEQVYREALKVVSSGAKEITLLGQNVNAYHGKGPDDKIFTLADLLGHLAQIPNLERLR YMTSHPIDMTDDLIKLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDRDYYFDIINRLRE TRPDIVLSSDFIVGFPGETDEDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQVPE HIKSERLTILQQELTAQQLAFNESCVGSIMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPN KSLLGKITDVKITKAASNSLTGEVI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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