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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP667208Yeast1mgQuote
EP667208E. coli1mgQuote
BP667208Baculovirus200ugQuote
MP667208Mammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas fluorescens (strain Pf0-1)
UniProt IDQ3K6B7
Gene NamemiaB; Locus:Pfl01_4950
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY SQLGRWRELKLANPDMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARI SKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD VIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTTSH PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGI CISSDFIVGFPGETEKDFEQTMKLIADVGFDFSYSFVYSQRPGTPAADLADDTPEELKKE RLNALQHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGELQGRTENNRIVNFRCDNPTLI GQFADVHIDAAQPHSLRGSLIQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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