Request QuoteCatalog Number: xP667000RLFSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP667000RLFYeast1mgQuote
EP667000RLFE. coli1mgQuote
BP667000RLFBaculovirus200ugQuote
MP667000RLFMammalian Cell200ugQuote

Protein Information

SpeciesRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
UniProt IDQ3IW81
Gene NamemiaB; Locus:RHOS4_36350; ORFs:RSP_3600
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMSEARKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKV YSDLGRLRPLKTAKPDLKIGVAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLERTE GGARVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFA RLMAEARGLVERGVREITLLGQNVNAWSNDGRGLGGLIRELARIDGLERLRYTTSHPNDM ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTS DFIVGFPGETEADFEATLDLIRAVGFGSAFSFKYSARPGTPAAEKPELPAEVCDARLQRL QALVTEQQRAAQMAMVGREVGVLYEKAGRLPGQMVGKSDHLHAVHVEDKAGQVGDLVRVR ITASAPNSLAGERLGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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