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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP670217Yeast1mgQuote
EP670217E. coli1mgQuote
BP670217Baculovirus200ugQuote
MP670217Mammalian Cell200ugQuote

Protein Information

SpeciesDehalococcoides ethenogenes (strain 195)
UniProt IDQ3Z6Q4
Gene NamemiaB; Locus:DET1385
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-418
Expression Tag6xHis
Purity>90%
AA SequenceMPGYYLWTIGCQMNQAESERLGRLFELWGYSLADKAEDAELVLVNSCVVREHAENKVINR LHILRKLKDKNPRLKIALTGCLVGQDIASVRKKFPFVDYIFQPGALPDWGEIPEGFILPL KPPVSASITIMQGCDNFCTYCIVPYRRGREKSRSISELCCEAAELVRRGSREVVLLGQNV DSYGHDLPEKPCLADLLYALSDIPGLLRIRFLTSHPKDISQKLIDAMASLHKVCHSLSLP VQAGADTILAAMRRGYTSEQYRELVGRLKTAMPDISLQTDLIVGFPSETAEQFDQSYKLM SDIGYDAIHVAAYSPRPQTAAARDMADDVPVAEKKRRLKLIEDLQKETVSKANSALVDTF AEVLVEGRQKNKWQGRTLGGKLVFLESDLPLEGCLINVKIFKASPWSLQAKLVKILES
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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