Request QuoteCatalog Number: xP649043BMNSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP649043BMNYeast1mgQuote
EP649043BMNE. coli1mgQuote
BP649043BMNBaculovirus200ugQuote
MP649043BMNMammalian Cell200ugQuote

Protein Information

SpeciesBrucella abortus (strain 2308)
UniProt IDQ2YQS8
Gene NamemiaB; Locus:BAB1_2154
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDA DLVLLNTCHIREKASEKLYSALGRLRKMRDARAADGKELTIGVAGCVAQAEGQEILRRAP NVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHLPAPRREETRKRGVSAFLTV QEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG REWGLGELLFRLARIPGIARLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRI LKAMNRRHKADEYLRLIERIRNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQA YSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQYAFQDSMIGRKMDVLLEKPG REAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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