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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP705011GGUYeast1mgQuote
EP705011GGUE. coli1mgQuote
BP705011GGUBaculovirus200ugQuote
MP705011GGUMammalian Cell200ugQuote

Protein Information

SpeciesGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
UniProt IDQ5FPF1
Gene NamemiaB; Locus:GOX2009
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMTTAPTSPALPASSDTAPTGPAPRGLHVITWGCQMNVYDSARMADVLRPLGYGPVERPED ADMVILNTCHIRERATEKVFSELGRLRKIRDERMSNGADRTIIAVAGCVAQAEGEVILTR APYVDLVLGPQTYHKLPEMVARAARAGGAVIETDFPVEQKFDFLPTDAAPQTQGNLTAFL TIQEGCDKFCSFCVVPYTRGAETSRPVASVLAEARRMAESGVREITLLGQNVNAYHGDDG KGGSETLAGLVEQLAQIPGLGRIRYMTSHPRDVDQSLIDAHRDNPALMPFLHLPVQSGSD RILKAMNRGHTADEYRESVRKLREARPDLALSSDFIVGHPGETEEDFEATMQLVRDIGFA MAYSFKYSPRPGTPAAGQPMQVPEDVKDRRLAELQALLREQQDAFNADMVGTVQEILVTN RGRKPGQIAGRSPYLQPVHFDGPDHLIGSTVKVAITTRRTNSLGGTLIRETASAC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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