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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP652074Yeast1mgQuote
EP652074E. coli1mgQuote
BP652074Baculovirus200ugQuote
MP652074Mammalian Cell200ugQuote

Protein Information

SpeciesNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
UniProt IDQ2Y5J2
Gene NamemiaB; Locus:Nmul_A2692
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMAKKLYIKTFGCQMNEYDSKKMADVLRDAQHMEKTDDPAAADVILFNTCSVREKAQEKVF HDLGRVRHLKAANPDLLIGVGGCVASQEGAEIVKRAPYVDLVFGPQTLHRLPQMISTRQI TGRPQVDISFPEIEKFDHLPPARTEGVTAFVSIMEGCSKYCSFCVVPYTRGEEISRPLDD ILTEIAGLTNLGVKEVTLLGQNVNAYRGRMQYAEEGELADFALLLEYLHEIPGIERIRYT TSHPREFTPRLIEAYKASPKLVSHVHLPVQSGSDRILAAMKRGYTSLEYKSIIRRLRAAR PDISITSDFIVGFPGETEADFEATMKLIEAVNFDGSFSFIYSSRPGTPAAGLEDTTPHQV KLERLQRLQEKVELQAQAISVRMVGTTQRVLVEGLSRKDPGELSGRTDNNRVVNFPGSPE MIGKFAELKITAALSHTLRGENVRADDAPIEPEAAIFRRYGAGPDSIGNRDNLT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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