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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP650460Yeast1mgQuote
EP650460E. coli1mgQuote
BP650460Baculovirus200ugQuote
MP650460Mammalian Cell200ugQuote

Protein Information

SpeciesMagnetospirillum magneticum (strain AMB-1 / ATCC 700264)
UniProt IDQ2VYQ8
Gene NamemiaB; Locus:amb4463
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-438
Expression Tag6xHis
Purity>90%
AA SequenceMAKRLFVKTYGCQMNVYDSARMADVLAPLGYGPADHAEEADMVILNTCHIREKAAEKVFS ELGRLRKLQAAKAEAGGRMILAVAGCVAQAEGEEILRRAPFVDIVLGPQTYHRLPEMVAQ AARAGGAVLDTEFPAEPKFDFLPEPHAEGTSAFLSVQEGCDKFCTFCVVPYTRGAEYSRP AAAVLAEAATLAAGGVREITLLGQNVNGWHGGEGWGLGRLIRALAEVEGVERIRYTTSHP RDMDDELIRAHAELPQLMPFLHLPVQSGSDRILAAMNRGHDRDTYLRLVDKLKSACPDLA LSSDFIVGFPGESDADFEASMDLIRRVGFVQTYSFKYSPRPGTPAAAMETQVPEAVKDER LAQVQALLLDQTMRFNHACVGREMRILLDRPGRHAGQLLGRSPYMQPVHVKAAAHLIGTV VPLRITKVHPNSLEAVPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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