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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP648547MUGYeast1mgQuote
EP648547MUGE. coli1mgQuote
BP648547MUGBaculovirus200ugQuote
MP648547MUGMammalian Cell200ugQuote

Protein Information

SpeciesMoorella thermoacetica (strain ATCC 39073)
UniProt IDQ2RJG3
Gene NamemiaB; Locus:Moth_1112
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMVKARKTFKIITYGCQMNQRDSEMMADLLQDAGYEPVAREEEAGVIILDTCCVREKAENK VYGKLGQIEKLKSANPDLVIAVAGCMVQQPGVAEKIRQQAPYVDLLLGTGNLQELPQLIE EIKAMHRPRIVVGEQEGPVVEDLPRRRARGAQAFVTITYGCNNFCTYCIVPYVRGRERSR RPENIIKEVKELVDQGVIEVTLLGQNVNSYGRDLRDGINFAGLLERVNAVEGLKRIRYVT SHPRDFTPELVTTISRLDKVCEHVHLPVQAGSNRILELMHRGYTREHYLELVADLRRHIP GISLTTDLIVGFPGETEADFEDTLDLVARVQFDNAFTFMYSPRRGTEAATMPGQLPTAIK KERLKRLMELQNSISLAKNEALVGQEVEVLVEGPSKTDPDQLSGRTRTNKLIIFPGDQSL TGRLVRVRLTRAQTWLLKGEMVDG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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