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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP653093Yeast1mgQuote
EP653093E. coli1mgQuote
BP653093Baculovirus200ugQuote
MP653093Mammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio desulfuricans (strain G20)
UniProt IDQ30YS1
Gene NamemiaB; Locus:Dde_2378
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-464
Expression Tag6xHis
Purity>90%
AA SequenceMHERTFHIMTFGCQMNVNDSDWLARALEARGFTQVPEHEAAIYIINTCSVRDKPEQKVYS LLGRIRRETKNRRNVTVCVGGCVAQQIGKGFFKRFSQVRLVFGTDGAASAPQAIERLVQE PHARISLLDFSEEFPERDAGWENGEVPVSAYVNIMQGCNNFCAYCIVPYTRGRQKSRSSA AVLDECRTLVGNGAREITLLGQNVNSYGLDPHGDGTTFARLLHDVAAIPGLERLRFMTPH PKDIAGEVIEAFGALKNLCPRVHLPLQSGSDRVLKAMGRKYDMARYMDIVTRLKAVRPDI QITSDLIVGFPGETEADFEQTLEAMRTVPFVQSFSFIYSDRPGTRAEMLPGKLSREEKTA RLVRLQEVQNEYSEAALQAMVGKTVMVLFESPSPKSAAGSGTDAQNAAEESGRTASSWQG RDEHGFILNVHLPAPADLYGKIMPVTVTAARKHSLTGEPAGESC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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