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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP657944Yeast1mgQuote
EP657944E. coli1mgQuote
BP657944Baculovirus200ugQuote
MP657944Mammalian Cell200ugQuote

Protein Information

SpeciesSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251) )
UniProt IDQ30PR1
Gene NamemiaB; Locus:Suden_1746
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-435
Expression Tag6xHis
Purity>90%
AA SequenceMQGMKKLFIETLGCAMNSRDSEHMIAELSEKEGYETTNDLSSADLIIINTCSVREKPVAK LFSELGVFNKRKKEGAKIGVCGCTASHLGEEIIKRAPYVSFVLGARNVSKISEVLHKERA VEIDINYDESEFAFNDFRTSPYKAYINISIGCDKSCTYCIVPKTRGEEISIPTELILREA QKAVKGGAKEIFLLGQNVNNYGRRFSGEHEKVSFSELLRRLSKIDALERIRFTSPHPFHM DDEFIEEFSSNPKICKSMHMPLQSGSSKVLKDMKRGYTKEWFLNRVQKLRAMSPDVSIST DIIVAFPGESDEDFEETLSIMREVKFDQIFSFKYSPRPETEAEHFTNIVDEDVASSRLTT LQNLAVNILDEKNKTHLGKIYRVYFEDLNQDYYASGRSDNNLLIKVKGSDELLGEFRDVK ITDIGRTILSGEIVG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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