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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP666117Yeast1mgQuote
EP666117E. coli1mgQuote
BP666117Baculovirus200ugQuote
MP666117Mammalian Cell200ugQuote

Protein Information

SpeciesNitrobacter winogradskyi (strain Nb-255 / ATCC 25391)
UniProt IDQ3SMT7
Gene NamemiaB; Locus:Nwi_0014
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-477
Expression Tag6xHis
Purity>90%
AA SequenceMPESRMPPRKLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKA SEKVYSELGRLRAAKDQAARDGRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHL PQLLARAKTCGRALETEFPVADKFGFLPQPSRQAIRSRGISAFVTVQEGCDKFCTFCVVP YTRGAEASRPVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAG IPGIVRLRYSTSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYR RVIDRFRQARPDIAFSSDFIVGFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAA DMQETVSTADMDQRLVQLQNLIDSQQSAFNRTALGRTIDVLFDRAGRKPGQIVGRTAYLQ PAHVEASDAIIGQVLPVTVASLERYSLFGTLASKPTSGEPSNHAATGGAQFQTTAGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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