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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP663473Yeast1mgQuote
EP663473E. coli1mgQuote
BP663473Baculovirus200ugQuote
MP663473Mammalian Cell200ugQuote

Protein Information

SpeciesThiobacillus denitrificans (strain ATCC 25259)
UniProt IDQ3SFF1
Gene NamemiaB; Locus:Tbd_2706
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMKKIYIKTFGCQMNEYDSDKMADVLNAAEGLTPTSTPEDADVILFNTCSVREKAQEKVFH DLGRVRHLKQANPNLIIGVGGCVASQEGAAIVARAPFVDVVFGPQTLHRLPELIAQRRET GRAQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKYCTFCVVPYTRGEEVSRPFDDV IAEVANLAARGVKEITLLGQNVNAYQGALDEGGTADFAFLLEMVHEIPGVERIRYTTSHP REMTQRLIECYGKLPKLVSHLHLPVQSGSDRILAAMKRGHTVLEFKSIVRKLREQRSDLC LSSDFIVGFPGETGADFEATMKLVEELNFDASFSFIYSKRPGTPAADYADDVSAELKTQR LMRLQARIEAQAQGVNRSMVGSVQRVLVEGQARKNANELAGRTGNNRIVNFAGPSRLLGQ FVDVTITQALPHSLRGEAVTLETLHT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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