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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP638057Yeast1mgQuote
EP638057E. coli1mgQuote
BP638057Baculovirus200ugQuote
MP638057Mammalian Cell200ugQuote

Protein Information

SpeciesBaumannia cicadellinicola subsp. Homalodisca coagulata
UniProt IDQ1LU21
Gene NamemiaB; Locus:BCI_0065
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-451
Expression Tag6xHis
Purity>90%
AA SequenceMNLKLHIKTYGCQMNQYDSSKIVDLLKNTHKYQLTDIAEEADILLLNTCSIREKAQEKLF HQLGRWRRLKKINPNLIIGVGGCVASQEGSNIRERANYVDIIFGPQTLHRLPEMINHVRI TRSPVIDISFPEIEKFDCMPKPQAKGPTAFVSIIEGCDKYCSYCVVPYTRGIEVSRPCED ILLEINNLADQNVREIHLLGQNVNAYRGTRYGGGTCTFAELLRLVAAINGIDRIRFTTSH PIEFTDDLIDVYRDTPELVSFVHLPVQSGSDRILALMKRGHTVLEYKSIIRKLYAVRPSI QISSDFIVGFPGETEEDFRQTMNLISEVNFDMSFSFIYSPRPGTPAANMVDIVSQDEKKQ RLYILQECIRKQAMKFSQAMKGTVQCILVEGTSRKNIMHLSGRTENNRVVNFIGNNGMIG QFVNVEIIDVYSNSLRGELISNQEKKYTLSA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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