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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP627577Yeast1mgQuote
EP627577E. coli1mgQuote
BP627577Baculovirus200ugQuote
MP627577Mammalian Cell200ugQuote

Protein Information

SpeciesKoribacter versatilis (strain Ellin345)
UniProt IDQ1IQH5
Gene NamemiaB; Locus:Acid345_1874
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMPVSSDPKTFYIETFGCQMNFHDSEKVVGTLISQGYRQVETELDAGLILYNTCSIRDKAE QKVFHRLSEFRQLQKEGKRFAVLGCVAQQEGEKIFERAPHVSLVAGSASYRNLAEMLVQI ESGSQRITGLDDRETDQTFETEFTARGNAHRGYITIIEGCDKFCAYCVVPYTRGKERSRS AESVLREARQMADAGFTDVQLLGQNVNSYHDPSGTMTFAELLTAVGEITGIKRVRFTTSH PRDFTRDIVEAIDNHPTLCDHVHLPVQSGSSKVLREMFREYTREQYLERISWMKATKNRK LSITTDVIVGFPGETETEFEETLALLDHVQYDGVFSFKYSPRPNTPALKYIDTVPEQEKS RRLQILMEHQREIQRANYRKHIGETIEVMVEAENATRAQWIGRTSQNKTLNFTVPQTVQP EIGSYHQVLVTQAFPNSLVGELVG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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