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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP632437Yeast1mgQuote
EP632437E. coli1mgQuote
BP632437Baculovirus200ugQuote
MP632437Mammalian Cell200ugQuote

Protein Information

SpeciesRhodopseudomonas palustris (strain BisB18)
UniProt IDQ21C43
Gene NamemiaB; Locus:RPC_0468
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMTQPRKLHIKSFGCQMNVYDAQRMVDALAPEGFVETANADEADLVILNTCHIREKASEKV YSELGRLRVAKDEAALQGRRMNIAVAGCVAQAEGAEIIRRQPAVDVVVGPQSYHHLPQLL AEAARGGRALETEFPVDDKFGFLPPPQPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG AEVSRPVDKIVEDVRRLADNGVREITLIGQNVNAYHGDGPDGRPWPLGALLHHLAKIPGI VRLRYSTSHPRDVDQSLIDAHRDLPALMPFVHLPVQSGSDAILAAMNRKHSADDYRRLVD RFRSANPAIAFSSDFIVGFPGETDEDFAATLALVTQIGYAGAYSFKYSPRPGTPAAELQE TVSAAVMDERLVRLQELIDSQQAAFNAAVIGTTVEVLFERAARNPGQIVGRTAYLQPAHV MAAPDIIGQVLPVRIDSLERYSLIGELAAPQQHSGFATRSEDSPQSLPITTGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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