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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP626980Yeast1mgQuote
EP626980E. coli1mgQuote
BP626980Baculovirus200ugQuote
MP626980Mammalian Cell200ugQuote

Protein Information

SpeciesSilicibacter sp. (strain TM1040)
UniProt IDQ1GK98
Gene NamemiaB; Locus:TM1040_0185
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-440
Expression Tag6xHis
Purity>90%
AA SequenceMSAPKKLYIKTYGCQMNVYDSERMAETLGGQGYVETQTPDDADMILLNTCHIREKAAEKV YSELGRFKGLKAEKPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQSYHRLPEMEAKAG TGEKVLDTDFPEEDKFEKLKRRPKAKRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRPV DRVLREAEDLVERGVREITLLGQNVNAYHGAGPNGDMTLAQLIWELDKIDGLERIRFTTS HPNDMMDDLIEAHGTCKKLMPYLHLPVQAGSDKILKRMNRSHTAESYIRLIERIRAARPD ILISGDFIVGFPEETEEDFQATLDLVEEVKYGTAYSFKYSTRPGTPAAERAQVDPKEADD RLQRLQAVLTRQQREVQDSMVGRELGVLFEKAGRFAGQMVGKSDYLHAVHVADCDAKIGD LRRVRIVSSGANSLAGELID
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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