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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP619703Yeast1mgQuote
EP619703E. coli1mgQuote
BP619703Baculovirus200ugQuote
MP619703Mammalian Cell200ugQuote

Protein Information

SpeciesShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
UniProt IDQ12R22
Gene NamemiaB; Locus:Sden_0815
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLDEYQGYTLTDDATEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKKPGLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIDQVQR GEKVVIDVSFPEIEKFDRLPEPRADGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPQDD IILEIAQLAEQGVREVNLLGQNVNAYRGATHDDQICTFAELLRLVASIDGIDRIRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKAREGI QISSDFIIGFPGESAEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVSDEEKKQ RLAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTENNRVVNFEAPHTHIG SFVDVEIVDVYTNSLRGIFIRGEAEMDLRRSLRPADILAKHKQDDVLGVTQFTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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