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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP615559Yeast1mgQuote
EP615559E. coli1mgQuote
BP615559Baculovirus200ugQuote
MP615559Mammalian Cell200ugQuote

Protein Information

SpeciesPolaromonas sp. (strain JS666 / ATCC BAA-500)
UniProt IDQ12EH0
Gene NamemiaB; Locus:Bpro_1120
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMSKKVFIKTFGCQMNEYDSDKMSDVLHAAEGYEPTQNVDEADLILFNTCSVREKAQEKVF SDLGRVKHLKEKGVLIGVGGCVASQEGAAIIQRAPYVDVVFGPQTLHRLPDLLAKRARLN KPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVL VEVAGLADQGVKEVTLLGQNVNAYRGPMGDTAEIADFATLLEYVSDIPGIERIRYVTSHP NEFTQSLIDAYARLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLA MSSDFIVGFPGETEDDFNKMMKLIDDVGYDTSFSFIYSPRPGTPAAALHDDTPHEVKLRR LQHLQATIDDSVRAISASRLGTVQRILVEGASRKDPTELMGRTECNRVVNFKGQPRLIGQ MVDITITQATQRSLRGEVLTRDVAVQA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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