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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP640868Yeast1mgQuote
EP640868E. coli1mgQuote
BP640868Baculovirus200ugQuote
MP640868Mammalian Cell200ugQuote

Protein Information

SpeciesJannaschia sp. (strain CCS1)
UniProt IDQ28UJ8
Gene NamemiaB; Locus:Jann_0697
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-462
Expression Tag6xHis
Purity>90%
AA SequenceMIEVSAVAGYTPSVEISLTPREAPQMTKKLFVKTYGCQMNVYDSERMAEALGGEGYEQVN TPEGADMILLNTCHIREKAAEKMYSELGRLRPLRDANPDLKIGVAGCVAQAEGEEIMRRQ PLVDLVVGPQTYHRLPKMMEAVNAGEKALDTDFPEEDKFLNLPKARATRGPTAFLTVQEG CDKFCAFCVVPYTRGAEVSRSAERLMAEARDLVDRGVREITLLGQNVNAYHGAGDGGTWG LARLIREMAKIDGLDRIRFTTSHPNDMEDDLIAAHGDCPELMPYLHLPVQAGSDKILKAM NRKHTAADYIRLIDRIRDARPDLHLSGDFIVGFPGETEEDFQATLDLVETVGYGTAYSFK YSARPGTPAAERTGQVSEAEASDRLQRLQALLTQQQRAAQDAMVGRRVKVLFEKPGRNPG QMIGKSEYLHSVHVDGPDTLRGQIAEVEIAESKTNSLTGRLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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