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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP630552Yeast1mgQuote
EP630552E. coli1mgQuote
BP630552Baculovirus200ugQuote
MP630552Mammalian Cell200ugQuote

Protein Information

SpeciesDesulfitobacterium hafniense (strain Y51)
UniProt IDQ24X58
Gene NamemiaB; Locus:DSY1595
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMSITKVPKKVVTLAYGCQMSERDADTLTEISSQKGYVRSQELEQADLIIVNTCCVRESAE NKILGKIGELKHLKEANPQLKIAISGCMVQQPGALERLRKRAPHVDIWAGTHNIHEFQRL LEEAEEKGKVAEVWEKPRETQESVLLAAKGKLKAYVNISYGCNNFCTYCIVPHVRGRERS RQPEEILAEIRALVETGCREVTLLGQNVNSYGQDLDRAYDFADLLKDVDSIDGLWRVRFM TSHPKDLSDKLIETIAAGTHLCEHIHLPFQAGSDEILKGMNRKYTREYYLSRIAQIKVII PQVSLTTDIIVGFPGETEEDFEQTLELIRQVRYSQAFTFMYSKRSGTPAAQMAEQIPLDI KKRRLQQLITVQNAQSLAWRQEMIGKTCEVLVEGPSKSNPDRLTGRTRGYELVVFPGEAQ LIGTLVQVLIQDANSWTLFGECRADRH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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