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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP633760Yeast1mgQuote
EP633760E. coli1mgQuote
BP633760Baculovirus200ugQuote
MP633760Mammalian Cell200ugQuote

Protein Information

SpeciesRhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118)
UniProt IDQ21T29
Gene NamemiaB; Locus:Rfer_3365
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-452
Expression Tag6xHis
Purity>90%
AA SequenceMSKKVFIKTYGCQMNEYDSDKMSDVLGAAQGYEPTDNVEEADLILFNTCSVREKAQEKVF SDLGRVKHLKKKGALIGVGGCVASQEGAAIIERAPYVDVVFGPQTLHRLPQLLAERERLN RSQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVL VEVAGLADQGVKEITLLGQNVNAWRSRMIGAASALSSEMADFATLLEYVSDIPGIERIRY VTSHPNEFTPSLIAAYDKLPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTVRKLRAI RPDMALSSDFIVGFPGETEDDFSKMMKLIDDVGFDNSFSFIFSPRPGTPAANLHDDTPHE VKLRRLQHLQTVINDSIKRISESRLGTVQRILVEGASKRDSTELMGRTECNRVVNFCGQP RLIGQMVDVAITETRSFTLRGEVVTQNEVLTG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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