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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP625757Yeast1mgQuote
EP625757E. coli1mgQuote
BP625757Baculovirus200ugQuote
MP625757Mammalian Cell200ugQuote

Protein Information

SpeciesMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
UniProt IDQ1H3L6
Gene NamemiaB; Locus:Mfla_0651
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMNLPKRIFIKTFGCQMNEYDSSRMLDMLQAAEGMQTTDNPEEADVILLNTCSVREKAQEK VFSHLGRFAPLKEKNPDLVIGVGGCVASQEGESIVARAPYVDVVFGPQTLHRLPDMIESK RRTGISQVDISFPEVEKFDHLPPPRVEGAAAFLSIMEGCSKYCTFCVVPYTRGDEVSRPF DDILTEAIQLAEQGVKEITLLGQNVNGYRSETADGEMADLALLIEYLAEIPEIERIRYTT SHPNEMTPALIDCYARIPKLVSHLHLPVQAGSDRVLMNMKRNYTVLQYKSIIRKLRAARP DICISSDFIVGFPGETEQDFEATMKLMRDVGFDFSFSFIYSPRPGTPASYLPDDCSPEEK QSRLSRLQALNEAQGKAISASMVGSIQRVLIESVSAKRADELAGRTDNNRIVNFPGDTAL INQFVNVRITEALSHTLRGELV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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