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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP626125Yeast1mgQuote
EP626125E. coli1mgQuote
BP626125Baculovirus200ugQuote
MP626125Mammalian Cell200ugQuote

Protein Information

SpeciesSphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256) (Sphingomonas alaskensis)
UniProt IDQ1GRE9
Gene NamemiaB; Locus:Sala_2064
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMKSDSPKTFHVKSFGCQMNVYDGERMAEMLGAEGYVPAADGADADLVVLNTCHIREKAAE KVYSDIGRLKRDDGSTPTIAVAGCVAQAEGAEIARRAPSVDVVVGPQAYHRLPELIARAE RGEKAIDLDMPAESKFGALPKRSGNQRPTAFLTVQEGCDKFCTYCVVPYTRGAEISRPFA DLIAEARALVAGGVREITLLGQNVNAWIGEDAKGRAIGLDGLIRELAKEDGLERIRYTTS HPNDMTDGLIVAHGEVDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPD IAISGDFIVGFPGESEADFAATLDIVRATRYAMAYSFKYSRRPGTPAATMDDQIDEAVMN ERLQRLQALLNEQQQAFNEATVGRTTRLLLERKGKREGQLIGKSPWLQSVHVTAPGLAIG DMIDVRITSAGPNSLGAEPLMAIA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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