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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP643321SSGYeast1mgQuote
EP643321SSGE. coli1mgQuote
BP643321SSGBaculovirus200ugQuote
MP643321SSGMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain JA-2-3B'a (2-13) ) (Cyanobacteria bacterium Yellowstone B-Prime)
UniProt IDQ2JKY0
Gene NamemiaB; Locus:CYB_1683
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMAAPTYYTITFGCQMNRADTERMAGILESLGYVATEDELQADLVLYNTCTIRDNAEQKVY SYLGIQAQRKRKNPAIKLIVAGCVAQQEGEKLLRRVPELDLVMGPQYVNRLGDLLAQVEA GNQVVATDPVEIPEDITKPRRDSQVTAWINVIYGCNERCTYCIVPRVRGQEQSRQPQAIR AEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRYF TEELITTCAELPKVCEHFHIPFQAGSNEVLKRMRRGYTHERYRQIIQLIRQYMPEAAISA DAIVGFPGETEAQFQETLQLVQDIGFDQVNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQR LNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRTRGNRLVFFEGDPEELQGSLV PVRITATRAFSLTGEAVTVRASGP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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