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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP639580Yeast1mgQuote
EP639580E. coli1mgQuote
BP639580Baculovirus200ugQuote
MP639580Mammalian Cell200ugQuote

Protein Information

SpeciesFrankia sp. (strain CcI3)
UniProt IDQ2J771
Gene NamemiaB; Locus:Francci3_3518
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-540
Expression Tag6xHis
Purity>90%
AA SequenceMNGRSYEVRTFGCQMNVHDSERLCGLLESAGYSPVDPGGEADVVVFNTCAVRENADNRLY GNLGQLVPVKKGHPGMQIAVGGCLAQKDRAAILDRAPWVDVVFGTHNLHRLPVLLERARH NAAAQVEIAEALEVFPSSLPTRRASHHSAWVSISVGCDNTCTFCIVPSLRGRERDRRPGD VLAEVEALVAEGALEITLLGQNVNSYGRSLGDPGAFAKLLAACGRVDGLERVRFTSPHPR DFTDDVIEAMATTSNVCHQLHMPLQSGSDTVLRRMRRSYRRDRFLGIVERVRAAMPDAAI TTDIIVGFPGETEADFADTLDVVRAARFSGAFTFQYSPRPGTPAATMDAQVDRATVADRY TRLVALQDEISWAENRALVGRRVEVLVSEGEGRKDGATGRMSGRARDGRLVHFRADEAAS SSTGSGPRAGGAAPAVAVAVAPTVRPGDVVETVVTRAAPHHLTADGPLRSHRATRAGDAW ALGRDGDGGGAAAAQQPADGRPIVTLGIPSLRPSSPDPVGPASADAASAGADACCTPVRR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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