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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP613668RFJYeast1mgQuote
EP613668RFJE. coli1mgQuote
BP613668RFJBaculovirus200ugQuote
MP613668RFJMammalian Cell200ugQuote

Protein Information

SpeciesRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114) ) (Roseobacter denitrificans)
UniProt IDQ161G5
Gene NamemiaB; Locus:RD1_3921
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-441
Expression Tag6xHis
Purity>90%
AA SequenceMTAPKKLFIKTYGCQMNVYDSERMSEALVAEGYVETKTAEDADMILLNTCHIREKAAEKV YSELGRLKSLKADNPDLKLGVAGCVAQAEGEEIMRRQPAVDLVVGPQSYHRLPQMEARLR EGHKALDTDFPPEDKFEELKARPKARRAPSAFLTVQEGCDKFCAFCVVPYTRGAEVSRPV TRVLDEARDLVERGVREITLLGQNVNAYHGAGADGNEKTLAQLIWALNDIDGLERIRFTT SHPNDMQDDLIEAHRDCPKLMPYLHLPVQSGSDRILKRMNRKHTADSYLRLIERIRVGRP DILLSGDFIVGFPEETEADFQATLDLIEAVNYGYAYSFKYSTRPGTPAAERAQVDPVEAD ERLQRIQALITRQQQDIQQSMVGRDVSVLIEKPGRFEGQMVGKSEYLHAVHVDQCSAQIG DILPVRIVEAKRNSLAAVTLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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