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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP645368Yeast1mgQuote
EP645368E. coli1mgQuote
BP645368Baculovirus200ugQuote
MP645368Mammalian Cell200ugQuote

Protein Information

SpeciesAnaeromyxobacter dehalogenans (strain 2CP-C)
UniProt IDQ2IKE9
Gene NamemiaB; Locus:Adeh_2355
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-464
Expression Tag6xHis
Purity>90%
AA SequenceMSDLVPLSRKPAPAAGDPAPSPAAPPRKVYVHTFGCQMNESDSDRMVELLGRHAYARAAS ADEADLILLNTCAVREKAEQKLLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPY VDFVFGPDNIARLPEMVERARGERFAETGWMDSEEYVFPRADAEAARGRATAFVTAMKGC DNVCAFCIVPHTRGREVSRPFPDVVAECAALAAVGVREVTLIGQNVNSYGGGCTFADLLR RVAAVPGIDRIRFTTSHPHDLSGALVEVFRDEPKVMPHFHLPVQSGSDAVLRRMRRDYSV AEYLDRFDRLRAARPGIAITTDFIVGFPGETDADFEASLALLERARFEQSFSFVFSPRPH TVAAVRLGSAPEWQDVPRDVAVARLERLLAAQRRIAGEILAAELGKVVEVLVEGPSDEPG ERLGRTPENRVVHLTADEAAAPAGARVPVRITRAGGSSLSGTLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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