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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP642192Yeast1mgQuote
EP642192E. coli1mgQuote
BP642192Baculovirus200ugQuote
MP642192Mammalian Cell200ugQuote

Protein Information

SpeciesAnaplasma phagocytophilum (strain HZ)
UniProt IDQ2GJJ5
Gene NamemiaB; Locus:APH_0880
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMTRGLYIESYGCQMNVYDALMMEDLLRPVGYAAVSRPEDADIILINTCHIREKASEKLYS TLGRMRVIKKEECILIVAGCVAQAEGEAVFERAPYVNVVVGPQGLHTLPELIMKVKRDKK QINIEFPVVSKFDAISIDRKANGGVSAYVSIQEGCDKFCTFCVVPYTRGPEYSRDVEAIL EEVKQLTDRGTKEIVLIGQNVNAYHGTYKGNEWDLGKLIQKVSLIDGVERIRYTTSHPRD MHPSLYEAHRDEKKLAPFVHLPVQSGSDAILRKMNRKHTAEEYLRVVEQLKDSNKNMALS SDFIVGFPGETEKDFEETMKLVESVGFALSYSFKYSPRPGTPGAEYSNQVPEEEKSARLA ALQGLLTKQQLQFNKSMEGRVMDVLVGDPSSMRSDRIFGKSEYTQSIHISAPSGSEDCFN RMVRVEILHGRQNSLEGTVLSNAN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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