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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP602293Yeast1mgQuote
EP602293E. coli1mgQuote
BP602293Baculovirus200ugQuote
MP602293Mammalian Cell200ugQuote

Protein Information

SpeciesGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
UniProt IDQ0BVY6
Gene NamemiaB; Locus:GbCGDNIH1_0118
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-493
Expression Tag6xHis
Purity>90%
AA SequenceMPMTGLLQTTLSTADQDRSGPAATTGDTPARANTKRLHVITWGCQMNVYDSARMTDVLSP LGYTAHAEPEGADMIVLNTCHIRDKATEKVFSELGRLRLLKEARAREGQPMLLAVAGCVA QAEGEQILARAPYVDIVLGPQTYHRLGGMVRRAMNGERVIETDFPPEDKFDYLPEAAAPQ HGPGLAGGRTAFLTIQEGCDKFCSFCVVPYTRGAEVSRPASSVLAEARRMVRGGAREITL LGQNVNAYHGLGEDGEVWTLARLIRALADIPDLLRIRYTTSHPRDVSDDLIAAHRDIPQL MPFLHLPVQSGSDRILAAMNRRHTAEDYFRVVDRLREARPDLALSSDFIVGHPGETDEDF EDTMRLIERVGFAQAYSFKYSPRPGTPAAERDNHVPEPVMDERLQRLQALLRTQQEAFNT ACIGKTVNVLLVTPGRHPGQIGGRSPWLQAVHLDAPATLIGQEVPVTITAAHTNSLSATL PDCAPHPKELTCA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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